Abstract
The heats of mixing of an equimolar mixture of poly G and poly C in solutions of various pH values were measured using a LKB batch type of microcalorimeter at 298±0.002 K. The interaction between poly G and poly C in neutral medium seems to lead to a complex such as poly(G+C). In order to obtain further information about the interaction between poly G and poly C, the CD spectra for poly G, poly C, and an equimolar mixture of poly G and poly C in solutions with various pH were also measured. From the results of the heats of mixing and the spectra measurements, it was concluded that poly G and poly C in neutral medium leads to a weak complex formation and the enthalpy of complex formation was stoichiometrically estimated to be about −9.3 kJ per mol of base pair.
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