Abstract
BackgroundTo study the healing processes of partial thickness wounds in the adult rabbit cornea after grafting a porous collagen-glycosaminoglycan copolymer matrix (CG).MethodsIn this study, the regeneration of surgically-induced rabbit corneal defect implanted with CG was investigated. The corneal partial thickness wound was created by 7.5 mm trephine. The wound was implanted with CG. Effects on wound healing was analyzed using clinical data on epithelial migration and corneal thickness, and histological data on collagen and alpha smooth muscle actin distribution.ResultsCompared with control group, CG induced a relatively severe inflammatory reaction in grafted cornea until the CG matrix was completely degraded. The new vessel ingrowth and stromal regeneration maintained the corneal thickness. The grafted cornea was significantly thicker (P < 0.001) than the control group. On day 90, the corneal opacity score of the control group was one and the grafted cornea was two.ConclusionCG copolymer matrix can successfully repair the damaged corneal stroma by injury, and regain its thickness. However, CG matrix induced inflammatory healing process thus causing mild corneal haziness and neovascularization.
Highlights
To study the healing processes of partial thickness wounds in the adult rabbit cornea after grafting a porous collagen-glycosaminoglycan copolymer matrix (CG)
Previous study demonstrated that CG matrix in eyes could modulate the healing procedure of conjunctival wound, reducing scarring contraction and promoting the formation of a near-normal subconjunctival stroma [9]
The staining for alpha-smooth muscle actin (α-SMA) was negative in both groups
Summary
To study the healing processes of partial thickness wounds in the adult rabbit cornea after grafting a porous collagen-glycosaminoglycan copolymer matrix (CG). Surgical procedures, including tissue adhesive [1, 2], amniotic membrane transplantation [1, 3], conjunctival flaps [1, 3], pericardial membrane graft [4], are done to temporarily maintain the integrity of the globe. This involves a multistage surgery [1].
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