Abstract
Objectives:To determine the effect of bone marrow-derived mesenchymal stem cells (BMSCs) on regeneration of bone marrow and intestinal tissue and survival rate in experimental mice with acute radiation syndrome (ARS).Methods:Forty mice were randomly divided into two equal groups of A receiving no BMSC transplantation and B receiving BMSCs. BMSCs were isolated from the bone marrow and cultured in DMEM media. Both groups were irradiated with 10 Gy (dose rate 0.28 Gy/ min) 60CO during 35 minutes with a field size of 35×35 for all the body area. Twenty-four hours after γ irradiation, 150×103 cells of passage 5 in 150 µl medium were injected intravenously into the tail. Animals were euthanized one and two weeks after cell transplantation. They were evaluated histologically for any changes in bone marrow and intestinal tissues. The survival rate in mice were also determined.Results:A significant increase for bone marrow cell count and survival rate were observed in group B in comparison to group A. Histological findings denoted to a healing in sample tissues.Conclusion:BMSCs could significantly reduce the side effects of ARS and increase the survival rate and healing in injured tissue. As such their transplantation may open a window in treatment of patients with ARS.
Highlights
Acute radiation syndrome (ARS) is a problem that can be caused due to exposure by high lethal doses of ionizing radiation during a short period of time causing depletion of parenchymal cellsCorrespondence: February 2, 2016 April 30, 2016 May 11, 2016 May 13, 2016 in a tissue.[1]
Culture of bone marrow-derived mesenchymal stem cells (BMSCs) in osteogenic media lead to osteogenic differentiation of the BMSCs based on presence of calcium deposits after three weeks of staining with Alizarin red (Fig.3)
The survival rate estimated by Kaplan-Meier test showed that exposure of whole body to 10 Gy γ irradiation and transplantation of BMSCs could significantly increase the survival rate in group B (Fig.4)
Summary
Acute radiation syndrome (ARS) is a problem that can be caused due to exposure by high lethal doses of ionizing radiation during a short period of time causing depletion of parenchymal cells. Correspondence: February 2, 2016 April 30, 2016 May 11, 2016 May 13, 2016 in a tissue.[1] High doses of ionizing radiation can cause detrimental systemic effects in organs such as the gastrointestinal tract, and blood circulation.[2,3] So, patients afflicted with this syndrome must i) prevented and treated for any infection,[4] ii) undergo hematopoiesis stimulation by administration of growth factors,[4] iii) receive stem cells[4,5] and iv) be protected physiologically from exposures.[4]. They have multilineage properties and can be differentiated into osteoblasts.[10]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
