Abstract
A haemolytic plaque assay for the enumeration of antibody forming cells in the carp ( Cyprinus carpio) is described. Serum of bream ( Abramis brama) turned out to be a more reliable complement source than allogeneic carp serum. The addition of 3–5% bream serum to a mixture of immune lymphoid carp cells and xenogeneic erythrocytes gave optimal results. Higher amounts of bream complement inhibited plaque formation. Plaque formation was also suppressed when inactive or heat-inactivated bream or carp serum was added to a mixture containing normal bream complement.
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