Abstract
The destructive processes which occur when red blood-cells are frozen and thawed have been observed. The results of these observations suggest that the principal damaging effect associated with freezing is the concentration of the electrolytes present within and without the cell. The destructive action of exposure to concentrated salt solutions is not instantaneous and the survival of rapidly frozen and thawed cells is attributed to this factor. The nature of the damage caused by exposure to strong salt solutions is complex. At moderate concentrations the blood-cell becomes sensitive to thermal and mechanical shock; at high concentrations the cell structure is dispersed by an apparently lyotropic effect.
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