The gubernaculum shows rhythmic contractility and active movement during testicular descent

Abstract

Androgens are postulated to act indirectly on the gubernaculum via the genitofemoral nerve, which contains specific neuropeptide transmitters. We examined gubernacula in vitro and in vivo to see if these neuropeptides affected the gubernacular development directly. Gubernacula (n = 272) from male rats (aged 0 to 3 days) were incubated in organ culture with various different neurotransmitters. The cultures were examined daily with a dissecting microscope connected to a video camera and tape. Some rats were anaesthetized and the inguinoscrotal skin was excised to expose the gubernacula for in vivo video recording. Of 181 gubernacula treated with calcitonin gene-related peptide (CGRP) at concentrations of 7 to 714 nmol/L, 92 showed rhythmic contractions. The number of responsive gubernacula was proportional to CGRP concentrations ( r = .685). This compared with 9 of 40 gubernacula treated with vasoactive intestinal peptide and 2 of 25 controls. Neither female gubernacula, skeletal muscle, nor umbilical cord showed CGRP-induced contractions. Gubernacula in vivo showed vigorous contractility and serpentine movements, which were accentuated by increased intraabdominal pressure. These studies demonstrate the gubernaculum to be highly motile during testicular descent. Rhythmic contractions suggest the presence of possible smooth muscle-like components that may be modulated by CGRP release from the genitofemoral nerve.