The growth of Synechococcus sp RDB001 at temperature of 30 ± 5 °C and 50 ± 5 °C: a comparison study of cell density and chlorophyll content

Abstract

Research on comparison of cell density and chlorophyll content of Synechococcus sp. RDB001 grown at a temperature of 30 ± 5 °C and 50 ± 5 °C has been carried out. Synechococcus sp. RDB001 was isolated from hot spring water samples in the area of Rawa Danau-Banten with a water temperature of 50 °C which grown in MA medium (pH 6). The research aims to determine the comparison of the average cell density and chlorophyll content of Synechococcus sp. RDB001 grown at a temperature of 30 ± 5 °C and 50 ± 5 °C in incubation cabinets. The research results are useful for understanding the physiological tolerance limits and adaptation of Synechococcus sp. RDB001 ex-situ. Culturing of Synechococcus sp. RDB001 was carried out in cabinets with the incubation temperature of 30 ± 5 °C and 50 ± 5 °C for 16 days, from day 0 (t0) until day 16 (t16). Each treatment was done in 16 replicates. Non-parametric statistical analysis using the Mann Whitney test (α = 0.05) and the Spearman test (α = 0,01). The results showed there were significant differences (α = 0.05) in cell density of Synechococcus sp. RDB001 grown at a temperature of 30 ± 5 °C and 50 ± 5 °C. The highest average of cell density of Synechococcus sp. RDB001 at a temperature of 30 ± 5 °C occurred at t12, (24.2075 ± 5.33926 x 106 cells.mL−1), while at a temperature of 50 ± 5 °C occurred at t14 (1.21313 ± 2.92573 x 106 cells.mL−1). There was no correlation (α = 0.01) between cell density and chlorophyll content of Synechococcus sp. RDB001 grown at a temperature of 30 ± 5 °C and 50 ± 5 °C. The research showed that increasing the cell density of Synechococcus sp. RDB001 is not always followed by an increase in chlorophyll content of Synechococcus sp. RDB001.