The growth of paprika (Capsicum annuum L.) from cotyledon on various media in vitro

Abstract

Micro propagation of paprika is still facing problems on suitable media composition. This research aimed to determine the growth of paprika (Capsicum annuum L.) from cotyledon on various media in vitro. The research was arranged as completely randomized design with 10 treatments, four replications, used Murashige-Skoog (MS) fortified with: 1). 3 benzylamino purine (BAP) + 0.3 indoleacetic acid (IAA) + 0.2 silver nitrate (AgNO3); 2). 3 BAP + 0.3 IAA + 0.3 gibberellin (GA) + 0.2 AgNO3; 3). 3 BAP + 0.3 GA + 0.3 naphthaleneacetic acid (NAA) + 0.2 AgNO3; 4). 3 BAP + 1.5 NAA + 3 AgNO3 + 1 glutamine; 5). 3 BAP + 0.5 NAA + 3 AgNO3 + 1 glutamine; 6). 4 BAP + 1.5 NAA + 3.5 AgNO3 + 1 glutamine; 7). 5 BAP + 1 IAA; 8). 10 BAP + 2 GA; 9). 5 thidiazuron + 2 GA; 10). 7.5 kinetin + 2 GA. All units are in mg/L. The treatment effects were observed on callus and shoot growth, analyzed by ANOVA and the mean differences were tested using Tukey Test. All treatments induced callus but only 10), 9) and 7) regenerated shoots of 3.75, 1.50 and 1,38 successivelly. Generally, addition of 7.5 mg/L kinetin + 2 mg/L gibberellin to Murashige-Skoog basal media was the best to induce shoot.