The growth, biochemical composition, and antioxidant response of Microcystis and Chlorella are influenced by Ibuprofen.

Abstract

Non-steroidal anti-inflammatory drugs like ibuprofen (IBU) are extensively used, causing substantial amounts to end up in aquatic ecosystems. Unfortunately, little research has been done on how these medications influence the physiology of phytoplankton. This study aimed to investigate the toxicological and physiological effects of IBU on the cyanobacteria Microcystis aeruginosa LE3 and Microcystis aeruginosa EAWAG 198, and the chlorophyte Chlorella sorokiniana. Exponential growth phase cultures were exposed to IBU at 10 to 10,000 μg/L for 96 h. The medium effect concentrations revealed varied sensitivity to IBU in the order Chlorella sorokiniana > Microcystis aeruginosa LE3 > Microcystis aeruginosa EAWAG 198. The drug caused a significant difference from control in cell density and chlorophyll-a of the three strains, except for chlorophyll-a in M. aeruginosa EAWAG 198 cultures where a significant difference occurred at 100 μg/L. The cell density of M. aeruginosa LE3 cultures exposed to 10 μg/L IBU increased 24 h post-exposure. Increasing concentrations of IBU induced higher total microcystins content of the Microcystis aeruginosa. Intracellular hydrogen peroxide content, peroxidase, and glutathione S-transferase activities, and lipid peroxidation increased as a function of IBU exposure. Total lipid, carbohydrate, and protein content of Chlorella sorokiniana were stimulated following IBU exposure. We conclude that the increasing presence of IBU in aquatic ecosystems could significantly alter the population dynamics of the investigated and other phytoplankton species.