Abstract
Summary Vesicular stomatitis virus (VSV) produces a cytopathogenic effect in roller-tube cultures of bovine tongue epithelium and guinea-pig kidney. Infectivity is first detected at about 15 hours by the formation of microscopic plaques of killed epithelial cells. Later the plaques increase in size and give the epithelial sheets a moth-eaten appearance. A titration-passage technique was devised to carry New Jersey type VSV in serial passage to a final dilution of 10-62. In this method each effective passage is made with a dilution of the preceding fluid which contains a minimal number of infectious units. The tissue culture virus retained its ability to infect chick embryos and to produce typical vesicular stomatitis in guinea pigs and cattle. The cytopathogenic activity of New Jersey type culture virus was neutralized by New Jersey immune but not by normal or Indiana immune guinea pig serum. Guinea pig cell clusters prepared by trypsinization were found to produce extensive coverage of the tube with cellular outgrowth. This technique was used for virus assay in which titers were read by color changes in the pH indicator as well as by cytologic change. Conventional serial passage of New Jersey type VSV in such cultures produced material with a tissue culture ID50 value of 106.2 per ml. Indiana type VSV extracted from infected bovine tongues gave tissue culture ID50 values of this same magnitude.
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