Abstract
Lectins have potential applications in agriculture, medicine, and biotechnological research and have become an important tool in the identification of different blood groups. Lectins can be obtained from different sources, such as microalgae. Tetradesmus obliquus is a green microalga that produces bioactives with high added value. A novel lectin was purified from microalgae T. obliquus by ion exchange and gel filtration chromatographies and characterized by molecular mass determination; carbohydrate contents; recognition of the rabbit, bovine, horse, and ABO blood type; inhibition by carbohydrate and glycoprotein; optimum pH and temperature; and their stability. Lectin was purified with a purification factor of 4.2-fold and yield of 3.13 in a two-step using ion exchange and gel filtration. It agglutinated human type O and rabbit erythrocytes but not bovine, horse, human erythrocytes types A1, A2, B, and AB. Lectin showed optimum pH at 7.0–7.5 and stability for 90 min, while the optimum temperature of 20–50 °C showed fairly good thermostability. The lectin is a glycoprotein with 5.37% of carbohydrate, an apparent molecular mass of 78 kDa as determined by gel filtration, and the activity was decreasing in the presence of ions and inhibited only by glycoprotein azoalbumin, azocasein, and albumin, characteristics that indicate the lectin from T. obliquus as unique among microalgal lectins.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
