The gonad growth of Anguilla bicolor bicolor induced by hormone combination of HCG, MT, E2, and antidopamine

Abstract

<p class="NoParagraphStyle" align="center"><strong>ABSTRACT</strong></p><p class="NoParagraphStyle" align="center"><strong> </strong></p><p class="NoParagraphStyle">The aim of the study was to stimulate eel gonad growth by intramuscularly injection with a hormonal combination of human chorionic gonadotropin (hCG), methyltestosterone (T), estradiol (E<sub>2</sub>) and antidopamine (A) meanwhile 0.9% NaCl solution was used as control. Estradiol concentration in the blood serum were measured by enzyme-linked immunosorbent assay (ELISA). Stimulation with hCG 20 IU/kg in combination with MT 3 mg/kg and 10 μg/kg antidopamine (hTA treatment) very effective for increased the growth of gonads with indicators gonadosomatic index (GSI) of 4.80%, hepatosomatic index 1.01% and concentration of E<sub>2</sub> 2.49±0.67 ng/mL were significantly different (P<0.05) than others treatment after ten weeks of application.</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Key words: hormone, gonad growth, maturation, Indonesian short finned eel</p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle" align="center"><strong>ABSTRAK</strong></p><p class="NoParagraphStyle"> </p><p class="NoParagraphStyle">Penelitian ini bertujuan untuk mengetahui pertumbuhan gonad yang distimulasi oleh kombinasi <em>human chorionic gonadotropin</em> (hCG), metiltestosteron (MT), estradiol (E<sub>2</sub>) dan antidopamin (A). Ikan sidat disuntik dengan hormon dan 0,9% NaCl sebagai kontrol secara intramuskular. Pengukuran konsentrasi hormon E<sub>2</sub> dalam serum darah dengan <em>enzyme-linked immunosorbent assay </em>(ELISA). Stimulasi dengan hCG 20 IU/kg dikombinasi dengan MT 3 mg/kg dan antidopamin 10 μg/kg (perlakuan hTA) sangat efektif untuk feminisasi dan meningkatkan pertumbuhan gonad dengan indikator indeks gonadosomatik (GSI) 4,80%, indeks hepatosomatik 1,01% dan konsentrasi E<sub>2 </sub>2,49±0,67 ng/mL, secara signifikan berbeda pada taraf P<0,05 dibandingkan perlakuan lain setelah sepuluh minggu aplikasi.</p><p class="NoParagraphStyle"> </p><p>Kata kunci: hormon, pertumbuhan gonad, maturasi, sidat</p>