Abstract
The glycosaminoglycan was isolated from an isotonic saline extract of dry, fat-free rat skin in better than 80% yield. The product was virtually homogeneous as judged by electrophoresis on paper at several pH values, by electrophoresis on sponge rubber, and by fractional precipitation of the calcium and sodium salts with ethanol. The material corresponded to hyaluronate in electrophoretic mobility, solubility of its salts in ethanol, and in its content of a single hexosamine, glucosamine.
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