Abstract
The glycol methacrylate (GMA) resin is an excellent embedding medium for high resolution light microscopy. Due to the hardness of the resin blocks, thinner sections can be made, allowing for greater resolution than the conventional paraffin sections. The main component of the popular Technovit 7100 (T7100) and Technovit 8100 (T8100) embedding media is GMA. Current formulations of embedding media allow serial sections to be made, especially when using Ralph glass knives in conjunction with a rotary microtome with a retraction return stroke. T7100 is used primarily for histological purposes while T8100 is designed for immunohistochemical (IHC) studies. This chapter details the processing, embedding, and sectioning procedures for the Technovit resins focusing on T7100. In addition, protocols for sectioning using disposable steel knives on a conventional rotary microtome are also included.
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