The Global Hemostatic Thrombodynamics Assay in Healthy Children

Abstract

Background: There is evidence that the concentrations of clotting and anticoagulant factors in children depend on age and differ from those found in adults. The results of APPT, TT, PT, fibrinogen are similar in children and adults in some studies, while PT and APTT show differences in others. Recent studies on global hemostatic assays Thromboelastography (TEG) revealed no significant differences in test results between healthy children and adults, while the thrombin generation test (TGT) showed significant differences. The Thrombodynamics (TD) assay is a new global hemostasis assay that considers the spatial organization of coagulation. Methods: APTT, TT, PR, fibrinogen and TD assays were performed in 102 healthy children between the ages of 1 and 17 years who underwent annual medical examinations and in 91 healthy adult volunteers. The following TD assay parameters were determined: lag time (Tlag), initial clot growth velocity (Vi), stationary clot growth velocity (Vs), clot size 30 minutes after the start of clot growth (CS) and clot Density (D). Written consent was obtained from all participants or their parents after they received complete information about the tests. Results: Age-specific reference values for the TD assay in healthy children aged 1-17 years are presented. No significant differences were observed between different age groups of children (15 years, 6-10 years, and 11-17 years) or between all children (1-17 years) and adults. Significant differences were not observed between genders. Conclusions: The TD assay results revealed no age-specific differences in the parameters between children aged 1-17 years and adults.