The genotype of Entamoeba histolytica in bloody diarrhea samples of humans, cows and sheep

Hassan H Naser

doi:10.33899/ijvs.2020.126135.1242

Abstract

The present study was carried out to detect the genotype of E. histolytica that found in human fecal specimens and animals feces with Haemorrgic diarrhea by amplifying the SREHP gene, using RT-PCR technique, Cyber green dye and by fusion curve analysis. The study also included molecular detection of amoebic parasite species using Nested-PCR technology. The study recorded presence of parasites E. histolytica; E. dispar; E. bovis with total infection rates 82.9, 26.8, 4.9%, respectively. The study revealed the presence of E. histolytica parasite in five different genotypes (I, II, III, IV, V) with rate presence 9.75, 53.65, 19.5, 9.75, 7.3%, respectively. In conclusion, there are five genotype of E. histolytica, in human and animals, most of these genotypes may be infect any host, E. bovis was recorded in sheep and cows.

Highlights

In the presence of E. histolytica and E. bovis as single infection indicating that these parasites can shift from a coexistence with the host to pathogenicity [5], many studies have returned that have attempted to differentiate between E. histolytica infection and its cause, from invasions of tissues into and out of the intestine and between the infectious of E. dispar and other parasites, even those studies were considered unsatisfactory [8]
This hypothesis is offset by recent studies that, using experimental animals, have demonstrated the events of the parasite E. dispar for focal ulcers and have an analytical capacity of the epithelial layer [9]. performance development of a parasite which isolated from a person with no symptoms of amoebiasis in a medium containing intestinal flora, it was observed endemicity of amoebiasis taking into account the overlap between the two parasites and pathogenic bacteria or intestinal flora, which may alter the behavior of these organisms into a nurse, since some pathogenic bacteria have genes that directly or indirectly encoded molecules activated by the inflammatory response [10]
The study showed the presence of the three parasites in the animals' samples, while the human samples were free from the presence of the parasite E. bovis (Table 8)

Summary

Introduction

Many species of amoeba genus were identified in the first quarter of the twentieth century [1] and are spread in various regions of the world in the developing countries of the Indian subcontinent, Central and South America, and in the tropics of Africa [2], as well as many species that parasitize many hosts, some of which are common to more than one host [3].The parasite has the ability to devour red blood cells by Erythrophagocytosis through the process of analyzing its plasma membrane and digesting the base material, as the ability of the feeding phases to digest erythrocyte may be due to the mechanism of adhesion to the surfaces of the cells and inhibit the adhesion of the parasite decreases phagocytosis and digestion of the erythrocytes during the parasite invasion of the large intestine layers [4], attributed [5] to (Hemoglobinase) and for the need of iron for the survival of the parasite, the digestion of blood hemoglobin to get iron [4,5]. In the presence of E. histolytica and E. bovis as single infection indicating that these parasites can shift from a coexistence with the host to pathogenicity [5], many studies have returned (and until recently) that have attempted to differentiate between E. histolytica infection and its cause, from invasions of tissues into and out of the intestine and between the infectious of E. dispar and other parasites, even those studies were considered unsatisfactory [8]. This hypothesis is offset by recent studies that, using experimental animals, have demonstrated the events of the parasite E. dispar for focal ulcers and have an analytical capacity of the epithelial layer [9]. This hypothesis is offset by recent studies that, using experimental animals, have demonstrated the events of the parasite E. dispar for focal ulcers and have an analytical capacity of the epithelial layer [9]. performance development of a parasite which isolated from a person with no symptoms of amoebiasis in a medium containing intestinal flora, it was observed endemicity of amoebiasis taking into account the overlap between the two parasites and pathogenic bacteria or intestinal flora, which may alter the behavior of these organisms into a nurse, since some pathogenic bacteria have genes that directly or indirectly encoded molecules activated by the inflammatory response [10]

Methods

Results

Conclusion