Abstract

Isolates of Apple chlorotic leaf spot virus (ACLSV) were collected from three species of pear (genus Pyrus) growing in Shandong and Hubei Provinces, China. The complete genome of one isolate (JB) and near-full-length genomes (30 nucleotides at 5′ end excluded) of two isolates (KMS and YH) were determined. These three isolates showed an overall nucleotide identity of 87.3–100%, and had the highest identity of 83.0% to an isolate MO-5 infecting apple in Japan and 74.9–75.8% identity to two recently reported ACLSV isolates from peach plants grown in China. The three ACLSV isolates from pear were mostly similar to ‘B6 type’ by having signature sites (S40-V59 or M59-Y75-T130-L184) in their coat protein. This is the first comparative study on the complete genomic sequences of ACLSV isolates infecting pear.

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