Abstract
High-coverage long-read sequencing of the Halobacterium salinarum type strain (91-R6) revealed a 2.17-Mb chromosome and two large plasmids (148 and 102 kb). Population heterogeneity and long repeats were observed. Strain 91-R6 and laboratory strain R1 showed 99.63% sequence identity in common chromosomal regions and only 38 strain-specific segments. This information resolves the previously uncertain relationship between type and laboratory strains.
Highlights
High-coverage long-read sequencing of the Halobacterium salinarum type strain (91-R6) revealed a 2.17-Mb chromosome and two large plasmids (148 and 102 kb)
The chromosomes of strains 91-R6 and R1 were compared in detail by methods we previously described for strain comparisons of Haloquadratum walsbyi and Photorhabdus laumondii [9, 10]
Given the close genomic similarity of the strains, the annotation of strain R1 was used as a reference for that of strain 91-R6
Summary
High-coverage long-read sequencing of the Halobacterium salinarum type strain (91-R6) revealed a 2.17-Mb chromosome and two large plasmids (148 and 102 kb). The chromosomes of strains 91-R6 and R1 were compared in detail by methods we previously described for strain comparisons of Haloquadratum walsbyi and Photorhabdus laumondii [9, 10].
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