Abstract
The grizzly bear (Ursus arctos ssp. horribilis) represents the largest population of brown bears in North America. Its genome was sequenced using a microfluidic partitioning library construction technique, and these data were supplemented with sequencing from a nanopore-based long read platform. The final assembly was 2.33 Gb with a scaffold N50 of 36.7 Mb, and the genome is of comparable size to that of its close relative the polar bear (2.30 Gb). An analysis using 4104 highly conserved mammalian genes indicated that 96.1% were found to be complete within the assembly. An automated annotation of the genome identified 19,848 protein coding genes. Our study shows that the combination of the two sequencing modalities that we used is sufficient for the construction of highly contiguous reference quality mammalian genomes. The assembled genome sequence and the supporting raw sequence reads are available from the NCBI (National Center for Biotechnology Information) under the bioproject identifier PRJNA493656, and the assembly described in this paper is version QXTK01000000.
Highlights
The grizzly bear is the most common sub-species of brown bears found in North America
A blood sample was taken as part of a routine physical exam of an adult male North American brown bear at the Toronto Zoo (GAN/ISIS:MIG12-29695490/34125)
A second genomic library was constructed from the same High molecular weight (HMW) DNA sample, but this one conformed to Oxford Nanopore Technologies’ protocols
Summary
The grizzly bear is the most common sub-species of brown bears found in North America. Brown bears (Ursus arctos) were historically found across much of North America, Asia, Europe, and even. As human encroachment on grizzly habitat continues, a better understanding of their biology will aid in conservation. DNA was sequenced from a 20-year old male grizzly bear, Samson. This bear was orphaned in the wilds of Alaska in 1998 when he was less than a year old. He was rescued and raised at the Alaska.
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