Abstract
Asparagus bean (Vigna. unguiculata ssp. sesquipedialis), known for its very long and tender green pods, is an important vegetable crop broadly grown in the developing Asian countries. In this study, we reported a 632.8 Mb assembly (549.81 Mb non-N size) of asparagus bean based on the whole genome shotgun sequencing strategy. We also generated a linkage map for asparagus bean, which helped anchor 94.42% of the scaffolds into 11 pseudo-chromosomes. A total of 42,609 protein-coding genes and 3,579 non-protein-coding genes were predicted from the assembly. Taken together, these genomic resources of asparagus bean will help develop a pan-genome of V. unguiculata and facilitate the investigation of economically valuable traits in this species, so that the cultivation of this plant would help combat the protein and energy malnutrition in the developing world.
Highlights
Background & SummaryAsparagus bean (Vigna unguiculata ssp. sesquipedialis, 2n = 2× = 22) is a warm-season and drought-tolerant subspecies of cowpea (Vigna unguiculata) with a wide cultivation area in East and Southeast Asia[1]
Unlike the grain-type subspecies common cowpea (Vigna. unguiculata ssp. unguiculata, or black-eyed pea), asparagus bean is harvested while its pod is still tender, thereby providing a very good source of protein, minerals, vitamins, and dietary fiber[4]
Three genetic maps were derived from either simple sequence repeat markers[12,13] or restriction-site associated DNA sequencing for asparagus bean[14]
Summary
Sesquipedialis, 2n = 2× = 22) is a warm-season and drought-tolerant subspecies of cowpea (Vigna unguiculata) with a wide cultivation area in East and Southeast Asia[1]. This plant is known as yardlong bean because of its characteristic pod that grows up to 50–100 cm in length[2]. As the DNA sequencing technologies became more advanced and affordable for the past decade, previous research had mainly focused on delineating the genome of common cowpea (estimated genome size of 620 Mb6).
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