Abstract

:Objective To analyzesequence characteristics and genetic polymorphisms of nine new Y-chromosomal short tandemrepeats (Y-STR) in Chaoshan Han population,and to evaluate their applications of forensicscience. Methods Blood samples of 159 unrelated male individuals from Chaoshan Hanpopulation were genotyped by PCR. The allele and haplotype frequencies of nine Y-STR lociwere calculated by the count ing method. Results Nine Y-STR loci (DYS522, DYS549, DYS556,DYS565, DYS568, DYS570, DYS594, DYS593, DYS588) were single copy, and 7, 5, 5, 4, 6, 9, 4,4, 8 alleles were detected respectively. The gene diversity (GD) value ranged from 0.1434to 0.7994. A total of 136 haplotypes were found, and the haplotype diversity was 0.997.None of Y-STR allele mutation was observed in the 30 father/son pairs with demonstratedpaternity. Conclusion The identifying power of 9 Y-STR loci are higher. Nine Y-STR locideveloped by us are suitable genetic markers for forensic applications.

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