The genetic map position of the locus encoding the 2S albumin seed storage proteins in cultivated sunflower ( Helianthus annuus L.)

Abstract

Sunflower contains two classes of seed proteins: the 11S globulins (helianthinins) and the 2S albumins. Polymorphisms have been detected within both classes and therefore they have been proposed as genetic markers. The objectives of the present study were to identify sunflower seed protein polymorphisms and locate them on an RFLP linkage map. Seed proteins of 68 inbred lines and four Argentinian open-pollinated populations were studied by SDS-PAGE. The helianthinins were separated into ten bands arranged in fourgroups of Mr of 22.5, 25.5, 32 and 38 kDa and all lines showed identical banding patterns. The albumins were separated into eight to tenbands in the Mr range 10 and 18 kDa. The lines showed either a band of Mr of 14.5 or of 15.5 kDa. HAR1, HAR2, ZENB8 and ZENB12 had the 15.5 kDa, whereas the remaining lines had the 14.5 kDa. The 14.5 and 15.5 kDa polypeptides were also segregating in the Impira INTA population. One hundred and fifty F2:3 families derived from ZENB8 × HA89 were used as the mapping population. The electrophoretic patterns of the families were either those of the two parental lines or presented both the 14.5 and 15.5 kDa polypeptides. No family showed absence of both bands, suggesting that they are allelic variants. The 2S albumin locus was located in the interval between the markers ZVG0051 and ZVG0052, at approximately 9 cM from ZVG0051 and at 6 cM from ZVG0052, on linkage group 11 of the public sunflower RFLP map.