Abstract
Cultivated strawberry (Fragaria × ananassa Duch.) is a high value horticultural crop. In this study, the genetic diversity of 160 strawberry accessions was determined using five highly polymorphic simple sequence repeat (SSR) markers. Sixty different alleles were identified, with allele frequencies in the range of 0.006 to1. Similarity scores were in the range of 0.034 to 0.963 (average: 0.507). The accessions were categorized into five groups. Group 1 contained two diploid Fragaria vesca species and one unknown accession. Group 2 contained one accession (F x ananassa). Group 3 contained 20 F × ananassa accessions and six unknown accessions. Group 4 contained 48 F. × ananassa accessions, one octaploid Fragaria chiloensis species, and six unknown accessions while Group 5 contained 69 F. × ananassa accessions and six unknown accessions. Accessions within a pedigree were frequently grouped together. A total of 30 novel accessions were categorized alongside existing accessions. These results will allow breeders to develop strategies which incorporate more genetic diversity into new cultivars.
Highlights
Cultivated strawberry (Fragaria × ananassa Duch.) is an octoploid (2n = 8x = 56) generated by natural hybridization between two octoploid strawberry species, F. chiloensis and F. virginiana (Hancock et al, 2010)
Information regarding the genetic diversity and population structure of breeding resources is important for efficient development of new cultivars
The genotyping results will provide a molecular basis for future breeding programs and will facilitate the development of novel strawberry cultivars with increased genetic diversity
Summary
The identification of strawberry cultivars was determined by the examination of differences in morphological or physiological characteristics such as leaf, flower, fruit parameters, and flowering habit (Dale, 1996; Nielsen and Lovell, 2000). Such methods are unsuitable for cultivated strawberries as genetic and phenotypic variations between cultivars are minimal. The completion of the genome sequence of diploid F. vesca (Shulaev et al, 2011) allowed robust SSRs to be developed and mapped on the Fragaria reference map (Rousseau-Gueutin et al, 2011; Sargent et al, 2011; Zorrilla-Fontanesi et al, 2011). The genotyping results will provide a molecular basis for future breeding programs and will facilitate the development of novel strawberry cultivars with increased genetic diversity
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