Abstract
Blackline disease of walnut, caused by the Cherry Leafroll virus (CLRV), causes fatal necrosis at the union between English walnut (Juglans regia) scions and hypersensitive rootstocks. `Paradox' hybrids between English walnut and Northern California black walnut (J. hindsii) are hypersensitive to CLRV. In 1983, `Paradox' trees were backcrossed to J. regia and J. hindsii. Patch graft inoculations of 111 backcrosses, the parent species and `Paradox' hybrids indicated that hypersensitivity to CLRV is inherited as a single dominant gene. Since breeding efficiency can be improved by the elimination of susceptible seedlings, we began a search for genetic markers tightly linked to CLRV resistance. DNA was sampled from the test population and from germplasm of both J. regia and J. hindsii. These samples were screened using randomly amplified polymorphic DNA (RAPD) markers and bulk segregant analysis. Over 360 random primers have been screened and two loci that are less than 10 map units from the hypersensitivity locus in this population identified. Efforts to find more closely linked markers that flank the hypersensitivity locus are continuing.
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