Abstract
Objective To study the inhibitory effect on macrophage migratory inhibitory factor (MIF) expression by MIF small interference RNA (siRNA)in human alveolar epithelial cell line A549. Methods After MIF siRNA was transfected into human alveolar epithial cells A549 with Interferin, RT-PCR was carried out to assess the dose-response of MIF siRNA's effect on MIF expression. Cell immumofluorescence method was used to analyze the expression of MIF protein. MTT cell proliferation assay was performed to detect the cytotoxicity of MIF siRNA to A549 cells, and the survival rate of A549 cells caused by lipopolysaccharide(LPS). Results (1) Only when the concentration was over 50 nmol/L, MIF siRNA significantly downregulated the MIF mRNA expression. (2) MIF siRNA also greatly reduced the expression of MIF fluorescent protein, which could be reversed by MIF. (3)MIF siRNA transfection had almost no toxicity to A549 cells, but also could reduce the cell mortality and increase the survival rate caused by LPS. Conclusions MIF siRNA can specially silence the expression of target gene MIF mRNA and reduce the MIF protein in A549 cells, therefore MIF siRNA may exert its safeguard to the death of cell induced by LPS. Key words: Macrophage migration inhibitory factor; Small interference RNA; Human alevolar epithelial cell A549; Lipopolysaccharide
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.