The GC/TOFMS based Serum Metabolomics in Rats with Depression-like Behavior after Exposure and Re-exposure to Chronic Unpredictable Mild Stress

Zhiguo Li,Shaojun Liu,Changqiang Zhu,Shuguang Yang,Xinghua Ding,Liang Jiao,Fangting Dong,Jianhua Chen,Yong Liu

doi:10.4172/2378-5756.1000396

Abstract

The Recurrent depression leads to disability and high health care costs. Identification of biomarkers for diagnosis recurrent depression will be helpful to predict and prevent the recurrence of depression. Sprague-Dawley rats were exposed and re-exposed to chronic unpredicted mild stress to mimic onset and recurrent depression. Rat’s serum was collected and analyzed by gas chromatography/time-of-flight mass spectrometry. Palmitinic acid and oleinic acid were found decreased in the onset depression and alanine, 6-Desoxy-Mannopyranose, oleinic acid, stearic acid, cholesterol was found decreased in recurrent depression. These data shows rats suffer recurrent depression have much more serious metabolic disturbance than onset depression, and they may provide valuable information for the potential biomarkers of distinguish onset and recurrent depression.

Highlights

Major depressive disorder (MDD) is ranked the second on a list of 15 major diseases in terms of burden in 2030 [1]
The t-test indicated after first Chronic Unpredictable Mild Stress (CUMS) regime, depression group showed significantly decreased sucrose preference (p
We identified the concentrations of palmitinic acid and oleinic acid were decreased in the serum of onset depression group

Summary

Introduction

Major depressive disorder (MDD) is ranked the second on a list of 15 major diseases in terms of burden in 2030 [1]. The major burden of MDD to disability and health care costs is largely due to its highly recurrent nature [2,3]. At least 50% of those who are recovered from the first episode of depression will have one or more additional episodes in their lifetime, and approximately 80% of those with a history of two episodes will have another recurrence [4]. The CUMS model can mimic several human depressive symptoms, and has good face validity, construct validity, predictive validity. This makes it is one of the most commonly used depression models which is suitable to study pathophysiology of depression [6]. The recurrent depression model is developed by exposing rats to CUMS again after they are recovered from the first CUMS-induced depression, and recurrence of depression is simulated [5]

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