The GABAergic anterior paired lateral neuron suppresses and is suppressed by olfactory learning

Abstract

GABAergic neurotransmitter systems are important for many cognitive processes, including learning and memory. We identified a single neuron in each hemisphere of the Drosophila brain - the anterior paired lateral (APL) neuron - as a GABAergic neuron that broadly innervated the mushroom bodies. Reducing GABA synthesis in the APL neuron enhanced olfactory learning, suggesting that APL suppressed learning by releasing the inhibitory neurotransmitter GABA. Functional optical imaging experiments revealed that APL responded to both odor and electric shock stimuli presented to the fly with increases of intracellular calcium and released neurotransmitter. More importantly, a memory trace formed in the APL neuron by pairing odor with electric shock. This trace was detected as a reduced calcium response in APL after conditioning specifically to the trained odor. These results demonstrated a mutual suppression between the GABAergic APL neuron and olfactory learning, and functional neuroplasticity of the GABAergic system due to learning.