Abstract
Abstract:The gene coding for a putative chlorophyll synthase gene (C4) from Arabidopsis thaliana was amplified by the polymerase chain reaction and cloned into the expression vector pQE‐ 31. Lysates of bacteria (E.coli) that had been transformed with this construct were used for in vitro enzymatic assays. The chlorophyll synthase catalyzed esterification of chlorophyllides a and b at the same rate but preferred geranylgeranyl‐PP over phytyl‐PP. This corresponds to the enzyme specificity previously described for etiolated plants and differed from that of green plants.
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