Abstract
Various geometric and densitometric parameters (area, projection, perimeter, integrated optical density, form factor, mean bound path, and fractional areas) were obtained by automated image analysis (Quantimet 720D) from Feulgen-stained WI38 human diploid fibroblasts, confluent (G0) and 3 h after nutritional stimulus (G0+G1). The frequency distribution of area and perimeter from the stimulated population show two distinct peaks (G0+G1) which are similar in appearance to fluorescence histograms previously reported following nutritional stimulus, while no significant differences existed between mean values for IOD (DNA content). The parameters versus optical density level threshold are consistent with the above findings, showing distributions indicative of chromatin dispersion during the G0-G1 transition.
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