Abstract
Three sets of Petunia hybrida Vilm. breeding lines with each set comprised of three genotypes, multiflora (gg), grandiflora (GG), and the heterozygote (Gg) were used in biochemical analyses to determine the possible mode of action of alleles at the G locus and their pleiotropic effects. Chlorophyll extractions did not correlate with visual observations that seedlings of the gg genotype have darker green foliage than seedlings of the GG genotypes. Likewise, chlorophyll, starch and sugar content were not related to greater seedling vigor, an apparent pleiotorpic effect to the g allele. Inconsistent differences in total soluble sugars and starch content were found with respect to the three genotypes. A qualitative difference in leaf sugar profiles as revealed by thin-layer and gasliquid chromatography separation was found. The Gg and gg genotypes consistently exhibited two extra bands at Rf 0.01 and at 0.52 to 0.56 which were absent in extracts from the GG genotype. The unknown compounds did not co-chromatograph with several sugar standards.
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