Abstract

The potential for wider use of two-dimensional liquid chromatography (2D-LC) is becoming more evident as the complexity of samples that must be handled continues to increase in application areas ranging from biopharmaceuticals to biosourced consumer products. Although the sophistication and ease of use have improved in recent years for commercial 2D-LC instruments, many analysts are still intimidated by the method development process for 2D methods because of the larger number of variables involved compared to conventional liquid chromatography. In this article, I share my perspective on the trends in this area, and the developments we are likely to see in the field in the near future.

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