Abstract
The letter from Drs. Lindeman, Visvader, Smalley and Eaves conveys their concern regarding the future of the prospective isolation and characterization of individual cells that may be characterized as mammary stem cells upon in vivo transplantation. As they have pointed out, the difficulties encountered in identifying specific mammary epithelial subtypes by different levels of fluorescence (particularly for membrane components that decorate most if not all mammary epithelial cells) leads to differential reporting and resultant confusion and underscores the need for improved standardization.
Highlights
The letter from Lindeman and coworkers [1] conveys their concern regarding the future of prospective isolation and characterization of individual cells that may be characterized as mammary stem cells upon in vivo transplantation
As they have pointed out, the difficulties encountered in identifying specific mammary epithelial subtypes by different levels of fluorescence leads to differential reporting and ‘resultant confusion’ and ‘underscores the need for improved standardization’
Our ‘re-evaluation’ [2] sought to highlight these concerns for those mammary and nonmammary biologists, who have been regaled in the current literature with glowing claims of the prospective isolation of normal and ‘cancer stem cells’
Summary
The letter from Lindeman and coworkers [1] conveys their concern regarding the future of prospective isolation and characterization of individual cells that may be characterized as mammary stem cells upon in vivo transplantation. See related letter by Lindeman et al, http://breast-cancer-research.com/content/10/3/402 and related review article by Smith and Medina, http://breast-cancer-research.com/content/10/1/203
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