The functionality characteristic of the different formulas of nano-emulsion containing temulawak-red palm oil extract

Abstract

Temulawak or Curcuma xanthorrhiza Roxb is usually used as traditional medicine (herbal medicine) by the community. Temulawak has antioxidant, anticancer, antihyperglycemic, anti-inflammatory, hepatoprotective and gastroprotective properties. Its application in functional foods is often limited by its low solubility and bioavailability in aqueous media. Nanoemulsion was made to improve the stability and bioavailability of Temulawak. Preparation of nano-emulsion was used high-pressure homogenization method. Temulawak extract in 10% red oil combined with Tween 20 and Tween 80 at a pressure of 1500 psi follows seven homogenization cycles. This method could produce nano-emulsion with the particle droplet below 150 nm. The antioxidant activity of temulawak extract nano-emulsion was 567.69 - 1324.62 ppm (AAE). The nano-emulsion preparation of temulawak extract was stable at 40°C storage, and its solubility increased (>90%). The in vitro bioaccessibility of temulawak extract nano-emulsion ranges from 23 to 98%. The optimum formula was produced with the concentration of 5% temulawak extract, 1% Tween 80, 2% Tween 20, with bioaccessibility of 98%. The optimum formula was tested in vivo bioavailability on white male rats with Spraque Dawley strain. The results showed that the nano-emulsion treatment had experienced faster elimination or metabolism than the temulawak extract. These results have important implications for effective delivery systems for bioactive compounds on temulawak.