Abstract
BackgroundIt has been reported that royal jelly would reduce melanin synthesis and inhibit the expression of melanogensis related proteins and genes. In this study, we evaluate the anti-melanogenic and depigmenting activity of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly of Apis mellifera.MethodsIn this study, we assesses the 10-HDA whitening activity in comparison with the changes in the intracellular tyrosinase activity, melanin content and melanin production related protein levles in B16F1 melanoma cells after treating with 10-HDA. Furthermore, the skin whitening effect was evaluated by applying a cream product containing with 0.5%, 1% and 2% of 10-HDA onto the skin of mice (C57BL/6 J) for 3 week to observe the effect of DL*-values.ResultsThe results showed that 10-HDA inhibited the MITF protein expression (IC50 0.86 mM) in B16F1 melanoma cells. Western blot analysis revealed that 10-HDA inhibited the activity of tyrosinase and the expression of tyrosinase-related protein 1 (TRP-1), TRP-2, and microphthalmia-associated transcription factor (MITF) in B16F1 melanoma cells. In addition, the 10-HDA was applied on the skin of mice show significantly increased the average skin-whitening index (L value).ConclusionsThe validation data indicated the potential of 10-HDA for use in suppressing skin pigmentation. The 10-HDA is proposed as a candidate to inhibit melanogenesis, thus it could be developed as cosmetics skin care products.
Highlights
It has been reported that royal jelly would reduce melanin synthesis and inhibit the expression of melanogensis related proteins and genes
The bioactive proteins of Royal jelly (RJ) are the major royal jelly proteins (MRJPs), apisimin and royalisin, which have shown immunoregulatory and antibacterial effects in several studies [4,5,6]. 10-hrdroxy-2decenoic acid (10-HDA) was the major fatty acid in RJ possess several health-beneficial effects for human, which has demonstrated antitumor, antibacterial, and immunomodulatory activities [7,8,9]. 10-hydroxy-2-decenoic acid (10-HDA) is only found in RJ so it has been used as a quality marker of royal jelly
Melanogenesis is a complex biosynthetic pathway controlled by tyrosinase, Peng et al BMC Complementary and Alternative Medicine (2017) 17:392 tyrosinase-related protein 1 and 2 (TRP-1 and TRP-2) and microphthalmia-associated transcription factor (MITF) [28, 29]
Summary
It has been reported that royal jelly would reduce melanin synthesis and inhibit the expression of melanogensis related proteins and genes. We evaluate the anti-melanogenic and depigmenting activity of 10-hydroxy-2-decenoic acid (10-HDA) from royal jelly of Apis mellifera. The bioactive proteins of RJ are the major royal jelly proteins (MRJPs), apisimin and royalisin, which have shown immunoregulatory and antibacterial effects in several studies [4,5,6]. 10-hrdroxy-2decenoic acid (10-HDA) was the major fatty acid in RJ possess several health-beneficial effects for human, which has demonstrated antitumor, antibacterial, and immunomodulatory activities [7,8,9]. To inhibit melanin synthesis via disruption melanogenesis would be the major way to prevent or improve hyperpigmentary disorders, such as melasma, and age spots. Searching for a potential, safe and effective compound to down-regulate in those factors in melanogenesis would be noteworthy in medical and cosmetic industry [25, 33, 34]
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