Abstract
Objective To investigate the effect of microRNA (miRNA, miR)-502-5p on colon cancer DLD-1 cells. Methods Colon cancer cell line DLD-1 was transfected by miR-502-5p mimic, control mimic, miR-502-5p inhibitor and control inhibitor respectively. The effect of miR-502-5p on the proliferation of DLD-1 cells was detected by cell counting kit-8 (CCK-8) assay. The effect of miR-502-5p on the colony forming ability of DLD-1 cells was detected by plate-cloning assay. Transwell assay was used to evaluate the effect of miR-502-5p on the migration and invasion of colon cells. Results The expression of miR-502-5p was significantly increased (319.31±33.16 vs. 1.08±0.22) in DLD-1 cells after transfection with miR-502-5p mimic (P 0.05), while migration [(621±90) cells vs. (174±32) cells] and invasion [(531±45) cells vs. (132±34) cells] ability were significantly increased (P 0.05), but the migration [(133±50) cells vs. (543±84) cells] and invasion [(119±45) cells vs. (458±57) cells] ability were decreased significantly (P<0.05). Conclusion MiR-502-5p may promote the migration and invasion of DLD-1 cells, but has no effect on the proliferation function. Key words: Colon cancer; MicroRNA-502-5p; Proliferation; Migration; Invasion
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