Abstract
Objective:To study the mechanism of the Wnt families'negative regulator cAxin2 in early inner ear development of chick embryo.Method: Plasmid was constructed with enhanced green fluorescent protein(EGFP), cAxin2 and short hairpin RNA(shRNA), which was transfected in otic vesicle by in ovo electroporation. Expression of cAxin2，cPax2(Pared box2)，and cBMP4(bone morphogenetic protein 4) genes was investigated in the transfected oticvesicle with situ hybridization.Result:cAxin2 expression was inhibited by shRNA，cPax2 was down regulated and cBMP4 was up regulated as well as an abnormally enlarged otic vesicle was discovered in the transfected otic vesicle. Conclusion:cAxin2 is an important regulatory gene required for inner ear development.
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