The Function and Prognostic Value of RNA-Binding Proteins in Colorectal Adenocarcinoma Were Analyzed Based on Bioinformatics of Smart Medical Big Data.

Haoran Zhao,Man Li,Yueyang Li,Yubao Zhang,Haishi Liu,Peng Wang,Zengfu Song,Qian Luo

doi:10.1155/2021/5536330

Abstract

Colon cancer is the third most frequent cancer in the world and is mainly adenocarcinoma in terms of pathological type. It has been confirmed that the dysregulation of RNA-binding proteins (RBPs) significantly participates in the occurrence and development of numerous malignant tumors. Therefore, we analyzed the RBPs associated with colon adenocarcinoma (COAD) to assess their possible biological effects and prognostic value. A total of 398 COAD tissue datasets and 39 normal tissue datasets were retrieved from the TCGA data resource and screened out the RBPs, which are differentially expressed between tumor tissues and nontumor tissues. Then, bioinformatics analyses based on smart medical big data were conducted on these RBPs. Overall, 181 differentially expressed RBPs were uncovered, consisting of 121 upregulated RBPs and 60 downregulated RBPs. Finally, we selected 7 prognostic-related RBPs with research prospects and constructed a prognostic model according to the median risk score. There were remarkable differences in OS between the high-risk and low-risk groups. In addition, the performance of the prognostic model was evaluated and verified with other COAD patient data in the TCGA database. The results showed that the area under the ROC curve (AUC) for the train group was 0.744 and the one for the test group was 0.661, confirming that the model assesses patients' prognosis to some extent. And based on 7 hub RBPs, we constructed a nomogram as a reference for evaluating the survival rate of COAD patients.

Highlights

Colorectal carcinoma is one of the most common gastrointestinal malignancies and the third most common cancer type in the world
1543 RNA-binding proteins (RBPs) were included, among which 181 RBPs were established as colon adenocarcinoma genes differentially expressed compared with normal samples
Molecular function (MF), biological process (BP), as well as cellular component (CC), the results of gene ontology (GO) enrichment analysis revealed that, in the BP gone through GO enrichment analysis, the downregulated RBPs were primarily involved in the modulation of mRNA processing and metabolic process, RNA localization and phosphodiester bond hydrolysis, modulation of translation, modulation of cellular amide metabolic process, while the upregulated RBPs were enriched during ncRNA processing and metabolic process, nucleic acid phosphodiester bond hydrolysis, and the biogenesis of ribosome and ribonucleoprotein complex (Table 1)

Summary

Introduction

Colorectal carcinoma is one of the most common gastrointestinal malignancies and the third most common cancer type in the world. E incidence of colon cancer is relatively high in western developed countries: taking the United States as an example, approximately 1,014,200 colon cancer patients are diagnosed each year [3]. As the global population is expanding and aging, colon cancer is expected to cause 60% more deaths by 2035 [4]. E onset of colon cancer is relatively insidious, and most of the patients had no obvious early symptoms. Colonoscopy is an effective method for screening colon cancer and removing precancerous lesions, which reduces the incidence and mortality of colon cancer [7]. Erefore, it is necessary to find an effective method for early screening, diagnosis, and prognosis evaluation based on the molecular mechanism of COAD Since colonoscopy is an invasive test requiring inconvenient preparation, colon cleansing, and laboratory test, it is not widely accepted by the target population. erefore, it is necessary to find an effective method for early screening, diagnosis, and prognosis evaluation based on the molecular mechanism of COAD

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