The full-length cDNA cloning and expression profiles of 14-3-3 genes from the mud crab Scylla paramamosain Estampador, 1949

Abstract

Two full-length cDNA sequences of14-3-3genes were isolated from the mud crab,Scylla paramamosainEstampador, 1949, using RT-PCR and RACE (rapid-amplification of cDNA ends). Sequence analysis indicates that both14-3-3genes contain an open reading frame of 744 bp with a deduced 247-amino-acid protein. The gene and protein sequences of Sp14-3-3genes show 96 and 97% identity, respectively, and both Sp14-3-3cluster together with other animal 14-3-3 proteins in phylogenetic tree analysis. Tissue specific expression analysis reveals that both Sp14-3-3genes are ubiquitously expressed, however, their expression patterns are different from each other. Expressions of both Sp14-3-3genes are sensitive to salinity decrease, even a 5 ppt drop from 30 ppt to 25 ppt, indicating that Sp14-3-3genes are involved in osmoregulation. Our observations may contribute to a better understanding of the molecular and functional evolution of the14-3-3family in both crustaceans and (in)vertebrates as a whole.