The four of structural genes sequences of a porcine epidemic diarrhea virus from domestic piglet in Fujian, China

Bo Dong,Xiaoyan Yang,Xiaohua Li,Ailing Dai

doi:10.1186/s12985-020-01345-7

Abstract

A prevalent PEDV strain, designated FJLY06, was isolated from Fujian, China. The four of structural genes sequences of PEDV obtained were analyzed to determine their phylogenetic relationships and homology respectively, revealing that FJLY06 was highly homologous to virulent PEDV strains. The four structural genes all differed genetically from the vaccine strain CV777. The sequence alignment results further showed that N, M and E genes of Chinese PEDV strains is highly conserved. Compared with the vaccine strain CV777, 8 mutations were detected in COE of FJLY06 S gene. The recombination analysis revealed FJLY06 is similar to other pandemic strains in China with a variant S gene, and maybe a reason for recent vaccination failures.

Highlights

Porcine epidemic diarrhea virus (PEDV) has four major structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins [1]
Analysis of a single structural gene may not really indicate genetic evolution because it reflects only a portion of the virus’s genes, in contrast, using four structural genes may be eliminate the bias of using a single gene for genetic phylogenetic analysis in PEDV
The E, M, N and S genes of this PEDV strain was cloned and sequenced to investigate the genetic characteristics and phylogeny of PEDV circulating in Fujian during this period. These data will provide a basis for further analysis of the genetic evolution of PEDV in China, and should help to develop a novel vaccine of PEDV for more effective prevention piglets from PEDV

Summary

Introduction

PEDV has four major structural proteins: spike (S), envelope (E), membrane (M), and nucleocapsid (N) proteins [1]. Since the 1990s, a periodic vaccination strategy has been placed into effect on pig farms to control PEDV in China.

Results

Conclusion