Abstract
Addition of OH − to air saturated dimethyl sulfoxide leads to the formation of the superoxide radical anion, as shown directly by electron paramagnetic resonance and ultra violet spectroscopy, and indirectly by superoxide dismutase inhibitable cytochrome c reduction. Superoxide production is related inversely to the water concentration of the dimethyl sulfoxide and solutions obtained are stable for up to three days. Reaction mechanisms are suggested and results are discussed in the light of the many uses of dimethyl sulfoxide as a solvent in both chemistry and biology.
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