Abstract
I T was shown in an earlier paper (James & Norval, I938) that the enzyme carboxylase is present in barley and that pyruvic acid supplied to living barley leaves and embryos is broken down, increasing their CO2 output and raising their R.Q. towards I-2. No pyruvic acid normally accumulates in the tissues, since negative results were consistently obtained with the iodoform and amnironia-nitroprusside reactions. This was attributed to the activity of the carboxylase system. Addition of pyruvate reduced the rate of oxygen consumption and the rate of reduction of methylene blue under nitrogen; oxidation of pyruvic acid therefore seems unlikely. It was the aim of the present work to see if pyruvic acid is formed, as distinct from accumulated. The added fact of its formation while respiration was going on would make the evidence of its taking a part reasonably complete for our species. Respiration is here taken to include glycolysis, whatever the relation between glycolysis and oxidation may be. METHODS
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
