Abstract
The first phosphorylated product formed from D-xylose by cell-free extracts of Pseudomonas hydrophila has been shown to be D-xylulose phosphate. This and other phosphorylated compounds derived from it have been identified by chromatography using specific color sprays, by spectrophotometric analysis, and by the formation of several pure, crystalline derivatives of the free sugars after dephosphorylation with acid phosphatase. The present data support the following initial pathway of D-xylose utilization (anaerobic): D-xylose [Formula: see text]D-xylulose → D-xylulose phosphate [Formula: see text]D-ribulose phosphate [Formula: see text]D-ribose phosphate [Formula: see text] sedoheptulose phosphate.
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