Abstract
Actinomyces and Leptotrichia formed a calculus-like deposit on single-rooted teeth incubated in a brain-heart infusion—thioglycollate broth. Polarized light, densitometric tracings and microscopic studies confirmed the similarity of the deposit to calculus formed in vivo. It is suggested that the role of Actinomyces and Leptotrichia in calculus formation is to enhance the rate of deposition of calcium salts.
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