Abstract
A simple mechanism for the sulphite-inhibited Maillard reaction of glucose and glycine has been critically appraised. By spiking mixtures of glucose, glycine and hydrogen sulphite ion (S(IV)) with 35S-labelled S(IV) or 3,4-dideoxy-4-sulphohexosulose (DSH), or 14C-labelled 3-deoxyhexosulose (DH), it has been shown that the rate determining step is the formation of DH and that, while S(IV) is present, the major reaction product is DSH. Once S(IV) has run out, DSH shows significant reactivity. The rates of formation and loss of DH are analysed in detail.
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