Abstract
During the enzymatic oxidation of uric acid by means of uricase (urate: oxygen oxidoreductase, E.C. 1.7.3.3) hydrogen peroxide is generated which in the presence of peroxidase 3,5-diacetyl-1,4-dihydrolutidine (DDL) oxidizes to substituted pyridine. Connected with this is a proportional decrease of fluorescence of the DDL. Maintaining the specificity of the uricase reaction, with the fluorescence measurement a higher sensitivity of uric acid determination is achieved than by applying spectrophotometric methods. Thus as little as 25 · 10 −12 moles of uric acid can still be determined. The application of the method to serum will be described.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
