Abstract
The cdc2 cell cycle control gene of Schizosaccharomyces pombe has been identified on a 3 kb DNA fragment. The gene is unique in the genome and is located near to a 5S ribosomal RNA gene. When a plasmid containing DNA sequences adjacent to the cdc2 gene is transformed into certain temperature sensitive cdc2 mutants it allows colony formation at the restrictive temperature. This was shown to be due to the plasmid interacting with the cdc2 chromosomal region and picking up the temperature sensitive allele of the cdc2 gene. Over expression of these temperature sensitive alleles presumably leads to sufficient activity of the thermolabile product to allow normal cdc2 function. In this way two cdc2 alleles have been cloned.
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