The first report of the prevalence of Nosema ceranae in Bulgaria.

Rositsa Shumkova,Ani Georgieva,Daniela Sirakova,Peter Hristov,Maria Bouga,Georgi Radoslavov,Gyulnas Dzhebir,Boyko Neov

doi:10.7717/peerj.4252

Abstract

Nosema apis and Nosema ceranae are the two main microsporidian parasites causing nosematosis in the honey bee Apis mellifera. The aim of the present study is to investigate the presence of Nosema apis and Nosema ceranae in the area of Bulgaria. The 16S (SSU) rDNA gene region was chosen for analysis. A duplex PCR assay was performed on 108 honey bee samples from three different parts of the country (South, North and West Bulgaria). The results showed that the samples from the northern part of the country were with the highest prevalence (77.2%) for Nosema ceranae while those from the mountainous parts (the Rodopa Mountains, South Bulgaria) were with the lowest rate (13.9%). Infection with Nosema apis alone and co-infection N. apis/N. ceranae were not detected in any samples. These findings suggest that Nosema ceranae is the dominant species in the Bulgarian honey bee. It is not known when the introduction of Nosema ceranae in Bulgaria has occurred, but as in the rest of the world, this species has become the dominant one in Bulgarian Apis mellifera. In conclusion, this is the first report for molecular detection of Nosema infection of honey bee in Bulgaria. The results showed that N. ceranae is the main Nosema species in Bulgaria.

Highlights

The Western honey bee (Apis mellifera L., Hymenoptera: Apidae) is a species of crucial economic, agricultural and environmental importance
We have presented for the first time molecular identification of two Nosema spp. and their distribution in Bulgaria
We found that N. ceranae infection prevailed in honey bee colonies from the flat part of the country (Rousse and Sofia district), while in the mountainous part (Smolyan district, the Rodopa Mountains) the prevalence was the lowest (Table 1)

Summary

Introduction

The Western honey bee (Apis mellifera L., Hymenoptera: Apidae) is a species of crucial economic, agricultural and environmental importance. The biological significance of bees is rooted in the fact that they are main pollinators in the natural environment (Barrios et al, 2016; Ballantyne et al, 2017). About 80% of the pollination of entomophilous plants is carried out by Apis mellifera. Active pollination significantly increases their yields (Partap, 2011). Honey bees are a valuable economic asset due to the ensemble of their products which includes honey, bee pollen, propolis, royal jelly, and bee venom, used by humans for food and treatment (Pasupuleti et al, 2017; Sforcin, Bankova & Kuropatnicki, 2017).

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