Abstract
This is the first report implementing combined density gradient centrifugation/Zeta (DGC/Zeta) sperm selection procedure for a couple with eleven previous intra cytoplasmic sperm injection/ in vitro fertilization (ICSI/IVF) failure cycles. Semen analysis was carried out according to World Health Organization criteria. Protamine deficiency, DNA fragmentation and morphology were assessed by chromomycin A3 (CMA3), TUNEL assay and papanicolaou staining, respectively. Patient was counseled regarding DGC/Zeta sperm preparation procedure. 10 oocytes were injected with combined DGC/Zeta sperm preparation which resulted in 90% fertilization rate and eight embryos with good quality. Three embryos were transferred on day three. Singleton pregnancy and healthy girl baby delivered with cesarean section. Result of this case report opens the horizon for further evaluation Zeta of sperm selection procedure for couples with repeated ICSI/IVF failure.
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