Abstract
AbstractHybrid recognition in the genus Fragaria (strawberry, Rosaceae) is difficult when based on morphological characteristics since there is great similarity among Fragaria species, even between different ploidy levels. This study aimed to identify and characterise an alloheptaploid hybrid (2n = 7x = 49) Fragaria for the first time in the genus from Europe (Germany, Bavaria) using different techniques. The hybrid was documented based on the evidence obtained from ploidy levels using flow cytometric measurement of nuclear DNA content, identification of the parental origin using 14 RAPD primers, fertility assessment from artificial crosses and fruit set level. RAPD analysis showed that the hybrid shared five species-specific markers with diploid F. vesca, and three with hexaploid F. moschata, indicating these species to be the most likely parents. The mean fluorescent intensity reflected by nuclear DNA in the putative hybrid (703) fell within the range accepted for heptaploidy in Fragaria, while the corr...
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