Abstract
Endosome-type vesicle membranes containing 50% cholesterol were prepared in the presence of 10-6 M of a β-tetrapyridiniumporphyrin. Strong fluorescence at 640 nm was observed. Addition of a slight excess (1.5 × 10-6 M) of copper(II) meso-tetraphenylsulfonate totally quenches the fluorescence in the bulk water volume. The vesicle-entrapped pyridinium porphyrins still fluoresce. In distilled water the residual fluorescence diminishes by less than 10% within 6 h, and in buffered solution it is reduced to 50% within 1 h. In the presence of a harpoon-like disruptor, almost quantitative quenching is observed within seconds (dipalmitoylphosphatidylcholine) or 10 min (egg lecithin).
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